Tobey Maguire...Peter Parker/Spider-Man
Kirsten Dunst....Mary Jane Watson
James Franco....Harry Osborn
Thomas Haden Church....Flint Marko/Sandman
Topher Grace....Eddie Brock
Bryce Dallas Howard....Gwen Stacy
Theresa Russell ....Mrs. Marko
Bill Nunn....Joseph 'Robbie' Robertson
Tanya Sinovec....Jazz Club Waitress
Dawn Marie Anderson....Nurse
Robert N. Anderson....Lab Technician
Terrell Anthony....Lab Technician
Elizabeth Banks....Betty Brant
Jodi Benker....Woman in Nightclub
Wilson Better....Chess Bully
Christina Cindrich....Lab Technician
Paul Terrell Clayton....Lab Technician
James Cromwell....Captain George Stacy
Tim De Zarn....Philip Watson
Jacob Head....Bouncer
Alex Kalognomos....Daily Bugle Photographer
Claudia Katz....Assistant
David Kilde....New Jersey Cop
Eugene Kim....Bar Patron
Mark Kubr....Bouncer
Tim Maculan....Director
Aimee Miles....Coffee Shop Waitress
Charles Moore....Businessman
Carolyn Neff....Lab Technician
Edward Padilla....Lab Technician
Brittany Paige....Nurse
Ted Raimi....Hoffman
Rogelio T. Ramos....Doctor
Cliff Robertson....Ben Parker
Bill E. Rogers....Chief State Trooper
J.K. Simmons....J. Jonah Jameson
Jason Paquin....Jazz Club Dancer (voice)
Tammi Sutton....Jazz Club Patron
Mageina Tovah....Ursula
Richard John Walters....Bartender
Ricarte Rivera....Jazz Club Dancer (voice)
January Welsh....Client
Jennifer Weston....Nurse
Toni Wynne....Bugle Employee
Dylan Baker....Dr. Curt Connors
Andy Bale....OsCorp Executive
Aliane Baquerot....Showgirl
Daniel Gillies....John Jameson (rumored)
Joe Manganiello....Flash Thompson
Lindsay McCoy....Motorcycle Onlooker
Laith Nakli....Man at news stand
Samantha Ressler....Samantha
Ronald Sylvers....Street Peddler
.skem9
